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Honey bee (Apis mellifera) is one of the most important pollinators of wild plants and agricultural crops. Chemically induced toxicity is the major cause of the recent extinction of bees that may result in a serious threat to global food security and planetary ecosystem services. In view of this, the registration and revaluation pipelines of regulatory agencies have required specific acute toxicity tests in honey bees. BeeToxAI can be used in integrative testing strategies as an alternative to animal tests for predicting acute toxicity of chemicals in honey bee.